It is not an industrial secrecy that most brand name products exhibit slightly acidic or basic surfaces. As the surfaces of these brand name columns are deactivated with either basic silanes or acidic silanes, these surfaces are bias inert to basic or acidic compounds. The methods and processes of surface deactivation employed by these companies lack of controllability, the surfaces are then not completely deactivated.
In other words, they are not totally inert. As a result of non-neutral surface, these brand name columns are more or less limited in certain applications, such as the analyses of amines, sulfurs, pesticides, environmental sample, and others. Non-neutral surface of a column not only produces inaccurate quantification but also sometimes produce small differences in separation and column selectivity. An example would be DB-5 columns are slightly different from HP-5 columns.
Abel has developed a proprietary surface deactivation technique to produce totally inert surface columns. In order to ensure the totally insert surface, AB columns are tested with toughest test sample contained base, acid and alcohol among industry standards. Table III lists the components of two test samples of Abel uses. |
| Table III Test sample components |
Column Brand |
AB-Inowax |
Brand H-Innowax |
AB-CarboWax
(true PEG column)
|
RI (Methyl Undecanoate)
|
1715.8 |
1716.2
|
1699.6
|
RI (1-Decanol)
|
1779.8
|
1779.7
|
1752.1
|
| K'Tetradecane) |
0.51 |
0.54 |
K'2,6-Dimethylphenol)
|
9.31
|
9.81
|
50 to 220≧ |
Temperature Limits
|
40 to 260/280≧
|
50 to 260/280≧ |
| Crosslinked and Bonded |
Yes |
Yes |
No |
Fig 3a and Fig 3b show inertness comparisons of most challenging surface, AB-50+ and Brand D-17 columns |
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In Fig 3a, 4-chlorophenol peak (the second peaks after solvents) exhibits slightly tailing, showing basic surface of brand D-17, that is a 0.53mm x 30m one. While in Fig. 3b, 4-chlorophenol peak is almost no tailing, showing better neutral surface of AB-50+, that is a 0.25mm x 30m one. It is clear that the brand D-17 column has lower peak height ratio of 4-chlorophenol over C14 than the AB-50+ column does.
Fig 4 demonstrates totally inert surface of AB-1 columns tested by AB-Ultra sample. Acid (4-chlorophenol), base (decylamine) and alcohol (1-decanol) exhibit symmetric peak shapes. Both AB-1 and AB-5 columns have best neutrally inert surfaces among all brand name columns, making it fit all applications. |
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